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Proceedings Paper

Open-source 3D-printed digital inline holographic microscope for low-cost cellular imaging
Author(s): Stephan Amann; Max von Witzleben; Stefan Breuer
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Paper Abstract

Digital inline holographic microscopy is a promising cellular object imaging modality. We report on two cost-efficient lens-less experimental set-ups comprising of a standard LED or a semiconductor laser light source with a Raspberry Pi Camera for image acquisition. The microscope parts are 3D-printed yielding a highly compact and portable microimaging solution. Tobacco cells, human red blood cells and polystyrene microspheres are successfully imaged by an open-source reconstruction software. The developed microscopes are cost-efficient (<$200) and yield spatial resolutions of 3.91 μm respectively 1.55 μm. They constitute a flexible tool for science and student and early researcher education that can be tailored to the researchers demand. All employed code is open-source accessible aiming at triggering further developments and sharing between research laboratories, diagnostic labs and science education.

Paper Details

Date Published: 21 February 2020
PDF: 9 pages
Proc. SPIE 11306, Practical Holography XXXIV: Displays, Materials, and Applications, 113060B (21 February 2020); doi: 10.1117/12.2547120
Show Author Affiliations
Stephan Amann, Technische Univ. Darmstadt (Germany)
Max von Witzleben, Technische Univ. Darmstadt (Germany)
Stefan Breuer, Technische Univ. Darmstadt (Germany)

Published in SPIE Proceedings Vol. 11306:
Practical Holography XXXIV: Displays, Materials, and Applications
Hans I. Bjelkhagen, Editor(s)

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