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Proceedings Paper

Quantification of tissue distribution and therapeutic response using paired agent imaging in a chicken chorioallantoic membrane xenograft assay (Conference Presentation)

Paper Abstract

Intracellular Paired-Agent Imaging (iPAI) quantifies intracellular drug targets using fluorescently-labeled small molecule imaging agents. iPAI has the potential to predict therapeutic response for individual patients but requires a patient derived xenograft (PDX) model that accurately mimics in vivo tumor heterogeneity yet is easily accessible for intravenous drug administration and rapid image collection. The chicken embryo chorioallantoic membrane (CAM) provides an intermediate, cost-effective model that provides vascularized, in vivo tumors in a matter of days (typically ~72 h) from implantation. Here, we investigate the implantation of thin (< 1 mm) cross-sectional slices (1-2 cm) of freshly excised tumor tissues from mouse xenografts. Multispectral iPAI will be performed to quantify the distribution and heterogeneity of drug targets within the tumor cross-section.

Paper Details

Date Published: 20 April 2020
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Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 112190E (20 April 2020); doi: 10.1117/12.2547100
Show Author Affiliations
Kimberley S. Samkoe, Dartmouth-Hitchcock Medical Ctr. (United States)
Lei G. Wang, Oregon Health & Science Univ. (United States)
Jesse R. Korber, Oregon Health & Science Univ. (United States)
Summer L. Gibbs, Oregon Health & Science Univ. (United States)
Kenneth M. Tichauer, Illinois Institute of Technology (United States)


Published in SPIE Proceedings Vol. 11219:
Visualizing and Quantifying Drug Distribution in Tissue IV
Kin Foong Chan; Conor L. Evans, Editor(s)

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