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Proceedings Paper

Indocyanine-green matching phantom for fluorescence-guided imaging system characterization and performance monitoring (Conference Presentation)

Paper Abstract

Fluorescence imaging for surgical guidance is a proven modality that allows for visualization of fluorescent markers in numerous biological imaging applications. As the field continues to develop there is an urgent need for fluorescence-imaging standards that enable system characterization and performance monitoring. Here, we present a long-term stable standard that mimics indocynine green (ICG) spectral behavior and is capable of probing the sensitivity of commercially available imaging systems. The standard is composed of three different tests: a varying concentration sensitivity test (0.1nM-3000nM), a tissue-equivalent-depth sensitivity test (0.2mm-7mm), and a 1951 USFA fluorescence resolution test. The fluorescence standard platform developed can incorporate 3-D printing with tissue-equivalent layers, enabling a wide variety of anatomical-specific designs. The manufacturing capabilities developed allow for incorporation of different fluorophores for applications beyond ICG and NIR.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11222, Molecular-Guided Surgery: Molecules, Devices, and Applications VI, 112220D (10 March 2020); doi: 10.1117/12.2547056
Show Author Affiliations
Alberto J. Ruiz, Dartmouth College (United States)
Mindy Wu, Duke Univ. (United States)
Ethan P. M. LaRochelle, Dartmouth College (United States)
Dimitris Gorpas, Technische Univ. München (Germany)
Joshua Pfefer, U.S. Food and Drug Administration (United States)
Vasilis Ntziachristos, Technische Univ. München (Germany)
Brian W. Pogue, Dartmouth College (United States)


Published in SPIE Proceedings Vol. 11222:
Molecular-Guided Surgery: Molecules, Devices, and Applications VI
Sylvain Gioux; Summer L. Gibbs; Brian W. Pogue, Editor(s)

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