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Proceedings Paper

Development of a time lapse tumor cell spheroid imaging system with a high-resolution spectral-domain optical coherence microscopy system (Conference Presentation)

Paper Abstract

Living cell culture provides convenient and standard biotechnology chosen option in the laboratory. However, current imaging methods could not present real 3D models. Therefore, we have developed a compact, high-speed spectral-domain optical coherence microscopy (SD-OCM) system to observe the interaction of the tumor cell spheroid with gold nanoparticles. Volumetric OCM images of the cell spheroid were acquired using an in-house C++ interface and used a low-cost microcontroller for triggering to synchronize the galvanometer mirror to the detector array. We designed a hermetic chamber on the microscope stage to control temperature, humidity, CO2 concentration in the experiment.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112431Q (9 March 2020); doi: 10.1117/12.2547049
Show Author Affiliations
You-Nan Tsai, National Taiwan Univ. (Taiwan)
Yu-Wei Chang, National Taiwan Univ. (Taiwan)
Chia-Chun Ni, National Taiwan Univ. (Taiwan)
Ting-Hao Chen, National Taiwan Univ. (Taiwan)
Ting-Yen Tsai, National Taiwan Univ. (Taiwan)
Ying-Peng Huang, National Taiwan Univ. (Taiwan)
Chih-Chung Yang, National Taiwan Univ. (Taiwan)
Hsiang-Chieh Lee, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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