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Proceedings Paper

Adaptive optics two-photon microscopy for in vivo imaging of mouse retina
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Paper Abstract

Non-invasive retinal imaging has greatly facilitated the research of eye disease and neurodegenerative disorders in the central nervous system (CNS). Two-photon microscopy is a powerful tool for in vivo imaging of mouse retina because it provides intrinsic optical sectioning capability and the infrared laser is less likely to excite the photoreceptors. However, the dilated mouse eye has large optical aberrations, which must be corrected to achieve high-resolution or even diffraction-limited imaging. Here, we developed an adaptive optics (AO) two-photon microscope for in vivo imaging of retinal neurons through the eyeball of living mouse. We used the two-photon excited fluorescence signal of retina as the guide star to measure and correct the aberration of mouse eye. After AO correction, the fluorescence signal was increased by at least fivefold and the fine structures such as axons of retinal ganglion cells (RGC) were clearly resolved. To take advantage of the non-invasive high-resolution imaging, we demonstrated functional calcium imaging of RGC responding to the light stimulations.

Paper Details

Date Published: 17 February 2020
PDF: 6 pages
Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 112480L (17 February 2020); doi: 10.1117/12.2546972
Show Author Affiliations
Zhongya Qin, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Sicong He, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Congping Chen, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Chao Yang, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jasmine Yung, The Chinese Univ. of Hong Kong (Hong Kong, China)
Wanjie Wu, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Christopher K.S Leung, The Chinese Univ. of Hong Kong (Hong Kong, China)
Kai Liu, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)


Published in SPIE Proceedings Vol. 11248:
Adaptive Optics and Wavefront Control for Biological Systems VI
Thomas G. Bifano; Sylvain Gigan; Na Ji, Editor(s)

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