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Proceedings Paper

Imaging cancer-associated fibroblasts in human breast tumor tissue using multiphoton microscopy
Author(s): Liqin Zheng; Wenhui Guo; Gangqin Xi; Jiajia He; Wenjiao Ren; Deyong Kang; Biying Yu; Chuan Wang; Jianxin Chen
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Paper Abstract

Fibroblasts are important players in proliferation, invasion, migration and metastasis of cancer cells. Until now, histological methods such as hematoxylin and eosin (H and E) staining are still a gold standard to assess fibroblasts in traditional histopathology. Multiphoton microscopy (MPM) has become a key technology for biological tissues imaging without staining at the cellular level. In this study, MPM based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) was used to identify the fibroblasts in breast cancer tissue. Normal fibroblasts with spindle shape and abnormal fibroblasts with stellate shape could be clearly obtained by MPM.

Paper Details

Date Published: 20 December 2019
PDF: 5 pages
Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112091G (20 December 2019); doi: 10.1117/12.2546962
Show Author Affiliations
Liqin Zheng, Fujian Normal Univ. (China)
Wenhui Guo, Fujian Medical Univ. Union Hospital (China)
Gangqin Xi, Fujian Normal Univ. (China)
Jiajia He, Fujian Normal Univ. (China)
Wenjiao Ren, Fujian Normal Univ. (China)
Deyong Kang, Fujian Medical Univ. Union Hospital (China)
Biying Yu, Fujian Normal Univ. (China)
Chuan Wang, Fujian Medical Univ. Union Hospital (China)
Jianxin Chen, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 11209:
Eleventh International Conference on Information Optics and Photonics (CIOP 2019)
Hannan Wang, Editor(s)

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