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Proceedings Paper

Characterization of single-molecule dwell times beyond photobleaching
Author(s): Thilini Perera; Wendi Fu; Ying Hu
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Paper Abstract

In this work we report a single molecule imaging method to spread the photon budget of a fluorophore over extended time periods by inserting a non-illuminating interval between consecutive frames. While photobleaching results in loss of fluorescent signals similar to the detachment of a fluorescently labeled biomolecules, we introduced this method to characterize the dwell times of single molecules.

Paper Details

Date Published: 13 February 2020
PDF: 5 pages
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124615 (13 February 2020); doi: 10.1117/12.2546920
Show Author Affiliations
Thilini Perera, Univ. of Illinois at Chicago (United States)
Wendi Fu, Univ. of Illinois at Urbana-Champaign (United States)
Ying Hu, Univ. of Illinois at Chicago (United States)


Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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