Share Email Print

Proceedings Paper

Rapid volumetric multiphoton microscopy via dual resonant scanning with deep learning (Conference Presentation)
Author(s): Chia Wei Hsu; Yu-Shin Hsu; Chun-Yu Lin; Shean-Jen Chen

Paper Abstract

A tunable acoustic gradient lens and a resonant mirror are integrated into a multiphoton excited microscope that is rapidly and precisely controlled by an embedded field programmable gate array to acquire images at 8 kHz for x-z plane. The pulse train signal of a femtosecond laser provides up to 95 MHz voxel rate, and one pulse per pixel. A volumetric image rate at up to 30 Hz can be realized according to the precise focal position and the excited signal, and deep learning. Biological images can be demonstrated at high-speed volumetric imaging rate.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441W (9 March 2020); doi: 10.1117/12.2546860
Show Author Affiliations
Chia Wei Hsu, National Chiao Tung Univ. (Taiwan)
Yu-Shin Hsu, National Chiao Tung Univ. (Taiwan)
Chun-Yu Lin, National Chiao Tung Univ. (Taiwan)
Shean-Jen Chen, National Chiao Tung Univ. (Taiwan)

Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?