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Proceedings Paper

Optimization of curcumin formulations for fluorescence-based applications
Author(s): Marcelo Saito Nogueira; Fabio Francisco Pinto Jr.; Raphael Antonio Caface; Kleber Thiago de Oliveira; Vanderlei Salvador Bagnato; Francisco Eduardo Gontijo Guimarães
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Paper Abstract

Curcumin is a natural compound used for medical and food-industrial applications due to its antioxidant, antiinflammatory, antifungal, antiviral, and antiseptic properties. In addition to these applications, curcumin can be used for fluorescence labeling in disease diagnostics/theranostics and monitoring photodynamic antimicrobial inactivation. In terms of fluorescence-based applications, the sensitivity to detect curcumin depends on the curcumin bioavailability or the formulation concentration. In this study, we used 375 nm and 445 nm laser excitation to characterize the wavelength dependent fluorescence spectra for curcumin in ethanol solutions of 10, 20, 50 and 75 μg/ml (i.e., 27.15, 54.30, 135.75 and 203.625 μM, respectively). Our results suggested the fluorescence intensity as a function of concentration saturates around 135.75 μM. Fluorescence intensity increase as a function of concentration was observed between 650 nm and 800 nm for the 445 nm excitation. Increase in the same wavelength range was obtained for the difference between the fluorescence spectra generated between the 375 nm and 445 nm excitation wavelengths. The spectral features reported in our study can be used on the design of curcumin formulations using Sigma Aldrich curcumin C7727 for fluorescencebased applications, determination of bioavailability of curcumin formulations and photodynamic dosimetry.

Paper Details

Date Published: 31 March 2020
PDF: 9 pages
Proc. SPIE 11238, Optical Interactions with Tissue and Cells XXXI, 112381F (31 March 2020); doi: 10.1117/12.2546823
Show Author Affiliations
Marcelo Saito Nogueira, Tyndall National Institute, Univ. College Cork (Ireland)
Fabio Francisco Pinto Jr., Univ. de São Paulo (Brazil)
Raphael Antonio Caface, Univ. de São Paulo (Brazil)
Kleber Thiago de Oliveira, Univ. Federal de São Carlos (Brazil)
Vanderlei Salvador Bagnato, Univ. de São Paulo (Brazil)
Francisco Eduardo Gontijo Guimarães, Univ. de São Paulo (Brazil)

Published in SPIE Proceedings Vol. 11238:
Optical Interactions with Tissue and Cells XXXI
Bennett L. Ibey; Norbert Linz, Editor(s)

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