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Proceedings Paper

Imaging Amphotericin B orientation in Candida spp. through polarization stimulated Raman scattering microscopy (Conference Presentation)

Paper Abstract

Candidemia remains the fourth most common cause of nosocomial bloodstream infections. For more than a half-century, amphotericin B (Amp B) has been the last line of defense in the treatment of life-threatening fungal infections. However, during the past several years, severe infections due to Amp B-resistant Candida spp. isolates have been increasingly reported. Here, through polarization stimulated Raman scattering microscopy, Amp B was found to accumulate largely in the cell membrane of Candida spp. in a highly orientated approach, and the interaction between Amp B and ergosterol was investigated as well. Moreover, we found that the correlation between Amp B and ergosterol in Amp B-susceptible Candida spp. is different from that of Amp B-resistant Candida spp., which provides us important information to understand the working mechanism of Amp B, and to achieve fast determination of the Amp B susceptibility of Candida spp.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125207 (10 March 2020); doi: 10.1117/12.2546819
Show Author Affiliations
Pu-Ting Dong, Boston Univ. (United States)
Yuewei Zhan, Boston Univ. (United States)
Zeina Dagher, Massachusetts General Hospital (United States)
Jie Hui, Boston Univ. (United States)
Kai-Chih Huang, Boston Univ. (United States)
Michael K. Mansour, Massachusetts General Hospital (United States)
Ji-Xin Cheng, Boston Univ. (United States)


Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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