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Proceedings Paper

Alignment-free frequency modulation stimulated Raman scattering microscopy (Conference Presentation)
Author(s): Andrew Hill

Paper Abstract

Stimulated Raman Scattering (SRS) microscopy is a powerful method for imaging molecular distributions based on their intrinsic vibrational contrast. However, SRS is hindered by a parasitic background signal which often overpowers the signal in low-signal applications. Frequency modulation (FM) has been used to suppress this parasitic background. However, many FM-SRS methods require the addition of multiple optomechanical components and extensive realignment. We report a new approach for alignment-free FM-SRS. In conjunction with a parabolically amplified Stokes pulse, we demonstrate near complete background suppression and also utilize the technique to increase the contrast of minority species in heterogeneous biological samples.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112520U (10 March 2020); doi: 10.1117/12.2546806
Show Author Affiliations
Andrew Hill, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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