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Proceedings Paper

In vitro quantification of single red blood cell oxygen saturation by femtosecond transient absorption microscopy (Conference Presentation)
Author(s): Andrew Francis

Paper Abstract

Blood oxygen saturation (sO2) plays an important role in maintaining energy homeostasis throughout the body. Clinical and research tools have been developed to monitor sO2 at a wide range of temporal and spatial scales. However, real-time quantification of sO2 at single red blood cell (RBC) resolution remains challenging. Such capability is critically important to study energy metabolism in heterogeneous tissues including brain and tumor tissue. In this work, we develop a label-free ratiometric transient absorption microscopy technique to image hemoglobin sO2. By exploiting differences in transient lifetime kinetics between oxyhemoglobin and deoxyhemoglobin, we directly quantified the sO2 of single RBCs.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112520M (10 March 2020); doi: 10.1117/12.2546786
Show Author Affiliations
Andrew Francis, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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