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Proceedings Paper

Interferometric imaging of neural activity (Conference Presentation)
Author(s): Daniel V. Palanker

Paper Abstract

Small cellular deformations associated with the changes of cell potential can reveal the underlying physiological activity. Using quantitative phase microscopy, we demonstrate deformations of up to 3nm during action potential in HEK cells, and ~1nm in primary cortical neurons. Phase-resolved OCT with adaptive optics in human eyes revealed rapid axial shrinkage of the cone outer segments, starting within 0.3ms after light stimulus, followed by a gradual expansion that saturates around 500ms. Characteristics of the rapid outer segment contraction are consistent with the early receptor potential, attributed to charge movement across the outer segment disc membranes during photoisomerization.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11253, Biomedical Applications of Light Scattering X, 1125309 (10 March 2020); doi: 10.1117/12.2546769
Show Author Affiliations
Daniel V. Palanker, Stanford Univ. (United States)


Published in SPIE Proceedings Vol. 11253:
Biomedical Applications of Light Scattering X
Adam Wax; Vadim Backman, Editor(s)

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