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Proceedings Paper

Photoinactivation of otopathogens using 405 nm blue light: Implications for the treatment of otitis media (Conference Presentation)
Author(s): Xiaojing Liu; Qihang Chang; Raquel Ferrer-Espada; Leon G. Leanse; Xueping S. Goh; Xiuli Wang; Jeffrey A. Gelfand; Tianhong Dai

Paper Abstract

Otopathogens such as Moraxella Catarrhalis and Haemophilus influenzae are the major causes of pediatric chronic and recurrent otitis media (OM). This pilot study showed that both M. catarrhalis and H. influenzae were highly susceptible to antimicrobial blue light (aBL) inactivation at 405 nm, either in suspensions and biofilms. Transmission electron microscopy showed aBL-induced damage of cell membrane in M. catarrhalis cells. Ultra-performance liquid chromatography results revealed that protoporphyrin IX and coproporphyrin are the most abundant species of endogenous porphyrins in M. catarrhalis. Our findings suggest that aBL is potentially an effective alternative antimicrobial therapy for OM.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230N (10 March 2020); doi: 10.1117/12.2546695
Show Author Affiliations
Xiaojing Liu, Wellman Ctr. for Photomedicine (United States)
Shanghai Skin Disease Hospital (China)
Massachusetts General Hospital (United States)
Qihang Chang, Wellman Ctr. for Photomedicine (United States)
Tongji Univ. (China)
Raquel Ferrer-Espada, Wellman Ctr. for Photomedicine (United States)
Massachusetts General Hospital (United States)
Leon G. Leanse, Wellman Ctr. for Photomedicine (United States)
Massachusetts General Hospital (United States)
Xueping S. Goh, Wellman Ctr. for Photomedicine (United States)
Massachusetts General Hospital (United States)
Xiuli Wang, Shanghai Skin Disease Hospital (China)
Jeffrey A. Gelfand, Massachusetts General Hospital (United States)
Tianhong Dai, Wellman Ctr. for Photomedicine (United States)
Massachusetts General Hospital (United States)


Published in SPIE Proceedings Vol. 11223:
Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
Tianhong Dai; Jürgen Popp; Mei X. Wu M.D., Editor(s)

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