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Proceedings Paper

In vivo quantification of mean corpuscular hemoglobin concentration by transient absorption microscopy and stimulated Raman scattering microscopy (Conference Presentation)
Author(s): Andrew Francis

Paper Abstract

Hematocrit, the volume fraction of red blood cells in whole blood, is a crucial metric of animal health in pharmacokinetic and disease model experiments. While robust, current methods for measuring hematocrit can be prohibitory for longitudinal animal models where animal well-being restricts serial blood collection volumes. Here we present in vivo hemoglobin concentration quantification in a mouse ear model using bimodal ratiometric imaging of transient absorption of hemoglobin and stimulated Raman scattering of water. Additionally, we leverage the intrinsic high resolution of nonlinear imaging to demonstrate a low volume method for ex vivo size and volume quantification.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125208 (10 March 2020); doi: 10.1117/12.2546665
Show Author Affiliations
Andrew Francis, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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