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Proceedings Paper

Advanced volumetric light field microscopy with temporal-focusing multiphoton selective excitation (Conference Presentation)

Paper Abstract

In this study, we implement temporal-focusing multiphoton selective excitation (TFMPSE) to light field microscopy (LFM), illuminating only the volume of interest, thus significantly reducing the background noise and providing higher contrast and accuracy for the light field image reconstruction; furthermore, offering higher penetration depth in scattering tissue via multiphoton. 3D human-skin in situ immunofluorescence images are used to demonstrate volumetric bioimaging capability. The volume rate of the TFMPSE-LFM can achieve around 100 volumes per second

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 112450G (9 March 2020); doi: 10.1117/12.2546629
Show Author Affiliations
Feng-Chun Hsu, National Chiao Tung Univ. (Taiwan)
Yong Da Sie, National Chiao Tung Univ. (Taiwan)
Chia-Yuan Chang, National Cheng Kung Univ. (Taiwan)
Chun-Yu Lin, National Chiao Tung Univ. (Taiwan)
Yvonne Y. Hu, National Cheng Kung Univ. (Taiwan)
Shean-Jen Chen, National Chiao Tung Univ. (Taiwan)

Published in SPIE Proceedings Vol. 11245:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Thomas G. Brown; Tony Wilson; Laura Waller, Editor(s)

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