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Proceedings Paper • Open Access

Spectral phasors by the sin-cos filter method in a two-photon excitation microscope (Conference Presentation)
Author(s): Enrico Gratton; Alexander Dvornikov

Paper Abstract

None of the methods used for hyperspectral imaging work for strongly scattering tissues. In this presentation we show that using the spectral phasor approach we could obtain the spectral phasor representation at each pixel of the image using two-photon excitation and a special filter in the emission that reproduce the sine and cosine transform that is needed to measure the spectral phasor in highly scattering tissues.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124404 (9 March 2020); doi: 10.1117/12.2546619
Show Author Affiliations
Enrico Gratton, Univ. of California, Irvine (United States)
Alexander Dvornikov, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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