Share Email Print
cover

Proceedings Paper

Immobilization of HIV GP41 antibodies on glass substrates for HIV biosensing
Author(s): Sello Manoto; Ahmed El-Hussein; Rudzani Malabi; Lebogang Thobakgale; Saturnin Ombinda-Lemboumba; Yasser Attia; Mohammed Kasem; Patience Mthunzi-Kufa
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Biological macromolecules such as antibodies, enzymes, proteins and aptamers have good molecular recognition ability which makes them good candidates for biosensing applications. In this study, glass substrates were treated with silane in order to immobilize HIV gp41 antibodies on their surfaces. The HIV pseudovirus was added to the treated substrates followed by addition of antibodies conjugated to nanoparticles. The surfaces were characterised by using water contact angle, atomic force microscopy (AFM) and Raman spectroscopy. Our preliminary data displayed that the antibodies were indeed immobilized on the glass substrates which made it possible for capturing the intact HIV pseudovirus. Further, Raman spectroscopy revealed the presence of disulphide bonds indicating successful conjugation of the HIV gp41 antibodies to the HIV pseudovirus.

Paper Details

Date Published: 21 February 2020
PDF: 9 pages
Proc. SPIE 11258, Frontiers in Biological Detection: From Nanosensors to Systems XII, 112580O (21 February 2020); doi: 10.1117/12.2546497
Show Author Affiliations
Sello Manoto, CSIR National Laser Ctr. (South Africa)
Ahmed El-Hussein, Cairo Univ. (Egypt)
Rudzani Malabi, CSIR National Laser Ctr. (South Africa)
Univ. of South Africa (South Africa)
Lebogang Thobakgale, CSIR National Laser Ctr. (South Africa)
Saturnin Ombinda-Lemboumba, CSIR National Laser Ctr. (South Africa)
Yasser Attia, Cairo Univ. (Egypt)
Mohammed Kasem, Cairo Univ. (Egypt)
Patience Mthunzi-Kufa, CSIR National Laser Ctr. (South Africa)
Univ. of South Africa (South Africa)
Univ. of KwaZulu-Natal (South Africa)


Published in SPIE Proceedings Vol. 11258:
Frontiers in Biological Detection: From Nanosensors to Systems XII
Amos Danielli; Benjamin L. Miller; Sharon M. Weiss, Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray