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Proceedings Paper

Fingerprinting individual bio-nanoparticles by interferometric photothermal microscopy (Conference Presentation)

Paper Abstract

Chemical characterization of biological specimens in the mid-infrared (IR) window plays a central role in the analysis of their functionalities. Although recent advances in mid-IR microscopy have demonstrated detection of the sample’s chemical contrast at a sub-micron resolution using a visible probe beam, they have limited sensitivity at high-throughput. To overcome this limit, we employ wide-field interferometric microscopy to detect the minute change in the optical path induced by mid-IR absorption. Our technique enables high-speed fingerprinting of more than thousands of sub-200 nm nanoparticles at once. This method paves the way for high-throughput, ultrasensitive, and label-free chemical imaging of individual bio-nanoparticles at sub-micron resolution.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521P (10 March 2020); doi: 10.1117/12.2546489
Show Author Affiliations
Celalettin Yurdakul, Boston Univ. (United States)
Haonan Zong, Boston Univ. (United States)
Yeran Bai, Boston Univ. (United States)
M. Selim Ünlü, Boston Univ. (United States)
Ji-Xin Cheng, Boston Univ. (United States)


Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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