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Proceedings Paper

Excitation-scan mirror array system advancements to hyperspectral imaging applications
Author(s): Marina Parker; Craig M. Browning; Sam A. Mayes; Joshua Deal; Samantha Gunn-Mayes; Thomas C. Rich; Silas J. Leavesley
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Paper Abstract

Hyperspectral imaging (HSI) technology has been applied in a range of fields for target detection and mixture analysis. While its original applications were in remote sensing, modern uses include agriculture, historical document authentications and medicine. HSI has shown great utility in fluorescence microscopy; however, acquisition speeds have been slow due to light losses associated with spectral filtering. We are currently developing a rapid hyperspectral imaging platform for 5-dimensional imaging (RHIP-5D), a confocal imaging system that will allow users to obtain simultaneous measurements of many fluorescent labels. We have previously reported on optical modeling performance of the system. This previous model investigated geometrical capability of designing a multifaceted mirror imaging system as an initial approach to sample light at many wavelengths. The design utilized light-emitting diodes (LEDs) and a multifaceted mirror array to combine light sources into a liquid light guide (LLG). The computational model was constructed using Monte Carlo optical ray software (TracePro, Lambda Research Corp.). Recent results presented here show transmission has increased up to 9% through parametric optimization of each component. Future work will involve system validation using a prototype engineered based on our optimized model. System requirements will be evaluated to determine if potential design changes are needed to improve the system. We will report on spectral resolution to demonstrate feasibility of the RHIP-5D as a promising solution for overcoming current HSI acquisition speed and sensitivity limitations.

Paper Details

Date Published: 17 February 2020
PDF: 7 pages
Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 112450Y (17 February 2020); doi: 10.1117/12.2546485
Show Author Affiliations
Marina Parker, Univ. of South Alabama (United States)
Craig M. Browning, Univ. of South Alabama (United States)
Sam A. Mayes, Univ. of South Alabama (United States)
Joshua Deal, Univ. of South Alabama (United States)
Samantha Gunn-Mayes, Univ. of South Alabama (United States)
Thomas C. Rich, Univ. of South Alabama (United States)
Silas J. Leavesley, Univ. of South Alabama (United States)

Published in SPIE Proceedings Vol. 11245:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII
Thomas G. Brown; Tony Wilson; Laura Waller, Editor(s)

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