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Proceedings Paper

High-sensitivity impulsive Raman spectroscopy with Doppler Raman spectroscopy (Conference Presentation)
Author(s): David R. Smith; Jeff J. Field; David G. Winters; Scott R. Domingue; Frauke Rininsland; Daniel J. Kane; Jesse W. Wilson; Randy A. Bartels

Paper Abstract

Doppler Raman (DR) spectroscopy is a coherent Raman technique that combines impulsive Raman excitation with novel frequency shift detection to enable high-sensitivity Raman spectroscopy in the biological fingerprint region (500cm-1-1500cm-1) and the low frequency regime from 10cm-1 – 500cm-1. Using DR, we demonstrate nonresonant Raman spectroscopy on a suite of biologically significant targets involved in cell respiration including cytochrome c, adenosine triphosphate (ATP), flavin adenine dinucleotide (FAD), and nicotinamide adenine dinucleotide (NADH). High-sensitivity detection of low-to-medium frequency Raman vibrational modes may provide a tool to monitor states of cell respiration along with large molecular structural changes such as protein conformational dynamics.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521Z (10 March 2020); doi: 10.1117/12.2546481
Show Author Affiliations
David R. Smith, Colorado State Univ. (United States)
Jeff J. Field, Colorado State Univ. (United States)
David G. Winters, Colorado State Univ. (United States)
Scott R. Domingue, Colorado State Univ. (United States)
Frauke Rininsland, Mesa Photonics, LLC (United States)
Daniel J. Kane, Mesa Photonics, LLC (United States)
Jesse W. Wilson, Colorado State Univ. (United States)
Randy A. Bartels, Colorado State Univ. (United States)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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