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Proceedings Paper

Nonlinear optical microscopy of lipogenesis and metabolism in HER2+ breast cancer (Conference Presentation)

Paper Abstract

Innate differences among breast cancer phenotypes are often studied from the perspective of single protein expressions or by fluorescent imaging using molecular tags. While these techniques can offer useful insights into breast cancer taxonomy, they do not easily translate to clinical care. Nonlinear optical microscopy has revolutionized our ability to study biochemical processes, as it offers a label-free approach to study differences in cancer phenotypes that may provide insight into factors affecting prognosis and treatment strategies. The endogenous chemical specificity offered by nonlinear imaging modalities like Stimulated Raman Scattering (SRS) and Multiphoton Fluorescence (MPF) are attractive alternatives to fluorescent imaging to study intracellular biochemistry. Here we utilize a multimodal imaging platform to characterize lipogenesis in HER2+ cell lines through SRS and metabolic activity by MPF of NADH/FAD to investigate unique signals related to HER2 status.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11216, Multiscale Imaging and Spectroscopy, 112160D (9 March 2020); doi: 10.1117/12.2546467
Show Author Affiliations
Sean Fitzgerald, Vanderbilt Univ. (United States)
Giju Thomas, Vanderbilt Univ. (United States)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 11216:
Multiscale Imaging and Spectroscopy
Paul J. Campagnola; Kristen C. Maitland; Darren M. Roblyer, Editor(s)

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