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Proceedings Paper

Correlative Brillouin microscopy and optical diffraction tomography for quantitative characterization of mechanical and biophysical properties of biological samples (Conference Presentation)
Author(s): Kyoohyun Kim; Raimund Schlüßler; Timon Beck; Salvatore Girardo; Jochen R. Guck

Paper Abstract

We present a combined optical system for Brillouin microscopy with optical diffraction tomography (ODT), which can reconstruct the three-dimensional refractive index (RI) distribution of biological samples. By correlating Brillouin frequency shift with the reconstructed RI distribution in the same field-of-view, we can calculate the precise longitudinal modulus of the samples without a priori information. We demonstrate the capabilities of the method using a cell phantom consisting of different hydrogel beads with known mechanical properties, and apply it to the quantitative characterization of the mechanical and biophysical properties of nuclear compartments inside individual cells under various physiological conditions including cell cycle progression and drug treatments.

Paper Details

Date Published: 11 March 2020
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Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490O (11 March 2020); doi: 10.1117/12.2546448
Show Author Affiliations
Kyoohyun Kim, Max-Planck-Institut für die Physik des Lichts and Max-Planck-Zentrum für Physik und Medizin (Germany)
TU Dresden (Germany)
Raimund Schlüßler, TU Dresden (Germany)
Timon Beck, Max-Planck-Institut für die Physik des Lichts and Max-Planck-Zentrum für Physik und Medizin (Germany)
TU Dresden (Germany)
Salvatore Girardo, Max-Planck-Institut für die Physik des Lichts and Max-Planck-Zentrum für Physik und Medizin (Germany)
TU Dresden (Germany)
Jochen R. Guck, Max-Planck-Institut für die Physik des Lichts and Max-Planck-Zentrum für Physik und Medizin (Germany)
TU Dresden (Germany)


Published in SPIE Proceedings Vol. 11249:
Quantitative Phase Imaging VI
Yang Liu; Gabriel Popescu; YongKeun Park, Editor(s)

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