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Proceedings Paper

High-throughput three-dimensional imaging cytometer for subnuclear foci quantification (Conference Presentation)
Author(s): Cheng Zheng; Dushan N. Wadduwage; Jong Park; Christy Chao; Jenny Kay; Norah Owiti; Zachary Nagel; Bevin P. Engelward; Peter T. C. So

Paper Abstract

We propose a high-throughput 3D imaging cytometer for fast quantification of DNA double strand break (DSB) frequency in cells for DNA damage study. With structured illumination enabled depth contrast and a fast focus tunable lens enabled scanning, this system generates a three-dimensional stack of clustered nuclei γH2AX foci with submicron resolution at a speed of 800 cells/second. Moreover, we unify the stack construction with the deep neural network, which largely improve quantification accuracy as well as the processing speed. Compared to previous 2D imaging approach, the addition of z-resolution in our 3D method provides an extra dimension of contrast and thus allows for more accurate DNA DSB quantification.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430X (9 March 2020); doi: 10.1117/12.2546302
Show Author Affiliations
Cheng Zheng, Massachusetts Institute of Technology (United States)
Dushan N. Wadduwage, Harvard Univ. (United States)
Jong Park, Massachusetts Institute of Technology (United States)
Christy Chao, Massachusetts Institute of Technology (United States)
Jenny Kay, Massachusetts Institute of Technology (United States)
Norah Owiti, Massachusetts Institute of Technology (United States)
Zachary Nagel, Harvard Univ. (United States)
Bevin P. Engelward, Massachusetts Institute of Technology (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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