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Proceedings Paper

Fast imaging of cardiomyocyte dynamics alterations after drug treatment utilizing quantitative phase digital holographic microscopy
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Paper Abstract

Digital holographic microscopy (DHM) is an interferometry-based variant of quantitative phase microscopy (QPM) that can be integrated modular into various common microscopes for label-free imaging of fast cellular morphology changes in a biomedical laboratory environment. We have utilized a fiber optics-based off-axis DHM concept to monitor the dynamics of beating cardiomyocytes after drug treatment. Our results show that local height changes due to cellular contractions can be detected spatially resolved at the subcellular level. Moreover, we demonstrate that a temporal resolution in the millisecond range is sufficient to detect drug induced increases and decreases of the beating rate.

Paper Details

Date Published: 14 February 2020
PDF: 5 pages
Proc. SPIE 11249, Quantitative Phase Imaging VI, 112491S (14 February 2020); doi: 10.1117/12.2546255
Show Author Affiliations
Björn Kemper, Westfälische Wilhelms-Univ. Münster (Germany)
Steffi Ketelhut, Westfälische Wilhelms-Univ. Münster (Germany)
Ivan Stojanovic, Saxion Univ. of Applied Sciences (Netherlands)
Martin L. Bennink, Saxion Univ. of Applied Sciences (Netherlands)
Peter Schön, Saxion Univ. of Applied Sciences (Netherlands)
Jürgen Schnekenburger, Westfälische Wilhelms-Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 11249:
Quantitative Phase Imaging VI
Yang Liu; Gabriel Popescu; YongKeun Park, Editor(s)

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