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Proceedings Paper

Frequency-tunable two-color ultrafast fiber laser for nonlinear excitation of NADH and FAD
Author(s): Axel Friedenauer; Konrad Birkmeier; Andreas Brodschelm; Max Eisele; Christoph Skrobol; Patrick Leisching
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Paper Abstract

We will present our latest innovations about ultrafast fiber lasers and show how multiphoton microscopy can benefit from these developments. Wavelengths around 900 nm and pulse durations as short as 100 fs remain a challenge for fiber lasers. Here we present a two-color femtosecond fiber laser system with synchronous outputs. One arm emits pulses at a central wavelength of 780 nm and the novel second laser arm is continuously tunable in its central wavelength between 810 nm and 950 nm. This allows the independent excitation of NADH and FAD and therefore enables optical metabolism and oxygen imaging of cells via FLIM and PLIM measurements.

Paper Details

Date Published: 14 February 2020
PDF: 4 pages
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441M (14 February 2020); doi: 10.1117/12.2546120
Show Author Affiliations
Axel Friedenauer, TOPTICA Photonics AG (Germany)
Konrad Birkmeier, TOPTICA Photonics AG (Germany)
Andreas Brodschelm, TOPTICA Photonics AG (Germany)
Max Eisele, TOPTICA Photonics AG (Germany)
Christoph Skrobol, TOPTICA Photonics AG (Germany)
Patrick Leisching, TOPTICA Photonics AG (Germany)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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