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Proceedings Paper

Optical imaging methods for label free detection of microplastics in cells, tissues and environmental organisms (Conference Presentation)
Author(s): Alvaro Barroso Pena; Steffi Ketelhut; Matthias Godejohann; Björn Kemper; Jürgen Schnekenburger

Paper Abstract

Microplastic particles are a major environmental pollution problem. Challenges in microplastics detection are the broad size range, chemical heterogeneity and low concentrations in liquids. Label free optical imaging methods for the detection and particle type identification were tested and compared. Quantitative Phase Imaging and holographic tomography were used to study cell response and cell uptake of microplastic particles. Optical Coherence Tomography could analyze larger organisms as Daphnia magna for microplastics uptake. Imaging methods combining particle localization with a chemical identification as RAMAN and mid IR spectroscopy could clearly identify different particle types and even detect specific microplastic chemistries inside cells.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 1124315 (9 March 2020); doi: 10.1117/12.2546098
Show Author Affiliations
Alvaro Barroso Pena, Westfälische Wilhelms-Univ. Münster (Germany)
Steffi Ketelhut, Westfälische Wilhelms-Univ. Münster (Germany)
Matthias Godejohann, MG Optical Solutions GmbH (Germany)
Björn Kemper, Westfälische Wilhelms-Univ. Münster (Germany)
Jürgen Schnekenburger, Westfälische Wilhelms-Univ. Münster (Germany)

Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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