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Proceedings Paper

Tracing the formation and degradation of fatty-acid-rich mitochondria using label-free chemical imaging (Conference Presentation)

Paper Abstract

Using coherent anti-Stokes Raman scattering (CARS) microscopy, we discovered heterogeneity of mitochondrial chemical compositions in cancer cells after hypothermia. Individual mitochondria associated with higher signals in CARS images are likely the result of fatty acid accumulation, which is caused by a reduced rate of fatty acid β-oxidation. Tracing individual mitochondria after reheating the cells to 37°C reveals degradation of these organelles through the mitophagy process. Further study will continue to unveil how stressed mitochondria would form in various conditions, and the fate of these organelles upon changes of their environment. Our results shed new light on mitochondrial function and cell metabolism.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521V (10 March 2020); doi: 10.1117/12.2546083
Show Author Affiliations
Chi Zhang, Univ. of Illinois (United States)
Stephen A. Boppart, Univ. of Illinois (United States)


Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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