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Proceedings Paper

Adaptive optics two-photon microendoscopy for high-resolution and deep-brain imaging in vivo
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Paper Abstract

Two-photon microscopy has become the method of choice for in vivo brain imaging in neuroscience research during the past decades owing to its inherent sectioning capability and large penetration depth in scattering tissues. By integrating with a gradient refractive index (GRIN) lens that implanted into the brain, two-photon microendoscopy further extends the imaging depth down to subcortical regions. However, the imaging resolution and field of view (FOV) are compromised due to the intrinsic aberrations of the inserted GRIN lens. Here, we developed an adaptive optics (AO) two-photon microendoscopy based on direct wavefront sensing that can measure and correct the aberrations of GRIN lens during in vivo brain imaging. Using our system, the diffraction-limited resolution was restored and the fine structures such as dendritic spines of hippocampal CA1 neurons can be clearly resolved over a much extended FOV.

Paper Details

Date Published: 21 February 2020
PDF: 6 pages
Proc. SPIE 11226, Neural Imaging and Sensing 2020, 1122606 (21 February 2020); doi: 10.1117/12.2546054
Show Author Affiliations
Congping Chen, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Zhongya Qin, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Sicong He, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Wanjie Wu, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Ye Wang, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Kam Fai Tam, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Nancy Y. Ip, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)


Published in SPIE Proceedings Vol. 11226:
Neural Imaging and Sensing 2020
Qingming Luo; Jun Ding; Ling Fu, Editor(s)

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