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Proceedings Paper

Electric field imaging with vibrationally-resonant electric field-induced sum-frequency generation
Author(s): Evan Perillo; M. Lisa Phipps; Jennifer S. Martinez; Anatoly Efimov
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Paper Abstract

We demonstrate all-optical sensing and imaging of quasi-DC electric fields using vibrationally-resonant electric field-induced sum-frequency generation (VR-EFSFG). Two femtosecond laser pulses at fixed 1035 and tunable 3500 nm are mixed in a cubically-nonlinear CH-rich sample to which an external field is applied by means of metallic electrodes defined via electron-beam lithography. The 2D images are acquired by raster scanning the lasers in an all-reflective laser scanning microscope. Photon-counting detection is implemented in transmission mode. Volt-level potentials across sub-micrometer gaps are imaged at a rate of approximately 0.5 frames per second. Signal enhancement of up to 50 times due to CH vibrational resonance is typically obtained, as verified by wavelength tuning of the mid-IR laser in the range 2400-3400 wavenumbers. Nearly ideal quadratic dependence of the signal on quasi-DC field amplitude is obtained confirming purely cubic nonlinear interaction in the sample. Using numerical modeling we establish the connection to imaging of transmembrane potential on neuronal axons and derive sensitivity limits of the method.

Paper Details

Date Published: 14 February 2020
PDF: 7 pages
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112442C (14 February 2020); doi: 10.1117/12.2546033
Show Author Affiliations
Evan Perillo, NanoString Technologies, Inc (United States)
M. Lisa Phipps, Los Alamos National Lab. (United States)
Jennifer S. Martinez, Northern Arizona Univ. (United States)
Anatoly Efimov, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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