Share Email Print
cover

Proceedings Paper

Spatial frequency projection imaging of coherent anti-Stokes Raman scattering (SPIFI-CARS) (Conference Presentation)

Paper Abstract

Conventional CARS microscopy requires scanning a point focus through the specimen limits imaging speed. We present a spatial frequency projection imaging (SPIFI) method for CARS microscopy to spatially multiplex CARS microscopy. A spinning disk modulator is used to rapidly modulate the Stokes field with a rapidly swept spatially periodic transmission grating. SPIFI-CARS images are obtained by Fourier transforming the single pixel signal. Images of CARS and second harmonic generation from histological slices will be presented. The physics of image formation and the impact of multiplexing on SNR will be discussed. Prospects for scaling to high speed CARS imaging will be discussed.

Paper Details

Date Published: 10 March 2020
PDF
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125203 (10 March 2020); doi: 10.1117/12.2546002
Show Author Affiliations
Sandro Heuke, Aix-Marseille Univ., CNRS (France)
Institut Fresnel (France)
Siddharth Sivankutty, Aix-Marseille Univ. (France)
Camille Scotte, Aix-Marseille Univ. (France)
Patrick A. Stockton, Colorado State Univ. (United States)
Randy A. Bartels, Colorado State Univ. (United States)
Anne Sentenac, Aix-Marseille Univ. (France)
Hervé Rigneault, Aix-Marseille Univ. (France)


Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray