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Proceedings Paper

In-vitro detection of polypeptide conformations at attomolar concentration using an ultrafast laser and surface-enhanced infrared absorption spectroscopy on a single resonant nanoantenna (Conference Presentation)
Author(s): Florian Mörz; Rostyslav Semenyshyn; Tobias R. J. Steinle; Monica Ubl; Mario Hentschel; Frank F. Neubrech; Harald Giessen

Paper Abstract

Infrared spectroscopy enables the investigation of conformational protein changes, associated with human diseases, such as Diabetes II or Alzheimer’s disease. However, the in-vitro investigation of individual molecules remains challenging, but could provide insight into mechanisms leading to structural changes. This is due to the lack of suitable light sources, among other things. Here, we detect polypeptide conformations at attomolar concentration within minutes, exploiting Fourier-transform infrared (FTIR) spectroscopy and the plasmonic enhancement of a single resonant nanoantenna, being enabled by using a highly brilliant, broadband mid-IR laser. We successfully determine polypeptide conformations and compare our results to Globar and synchrotron measurements.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11257, Plasmonics in Biology and Medicine XVII, 112570J (10 March 2020); doi: 10.1117/12.2545928
Show Author Affiliations
Florian Mörz, Univ. Stuttgart (Germany)
Rostyslav Semenyshyn, Univ. Stuttgart (Germany)
Tobias R. J. Steinle, Univ. Stuttgart (Germany)
Monica Ubl, Univ. Stuttgart (Germany)
Mario Hentschel, Univ. Stuttgart (Germany)
Frank F. Neubrech, Ruprecht-Karls-Univ. Heidelberg (Germany)
Harald Giessen, Univ. Stuttgart (Germany)

Published in SPIE Proceedings Vol. 11257:
Plasmonics in Biology and Medicine XVII
Tuan Vo-Dinh; Ho-Pui A. Ho; Krishanu Ray, Editor(s)

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