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Proceedings Paper

Alkyne-tag SERS imaging for visualizing small molecule drugs in live cells
Author(s): Kota Koike; Kazuki Bando; Jun Ando; Nicholas Smith; Kosuke Dodo; Satoshi Kawata; Mikiko Sodeoka; Katsumasa Fujita
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Paper Abstract

Raman microscopy is a powerful tool to observe molecular distribution in live cells. Here, we propose a technique to detect small molecule drugs using alkyne-tag surface-enhanced Raman scattering (SERS) imaging. To obtain SERS effect, we use gold nanoparticles as SERS probes so that we can enhance Raman scattering of small molecule drugs at low concentration with several orders of magnitude. We use alkyne tag to selectively detect the drug molecules by using the Raman peak of alkyne in the spectral silent region. Home-built slit-scanning Raman microscopy enables us to perform rapid SERS imaging. We successfully detect SERS signal from an alkyne-tagged inhibitor of a lysosomal enzyme with gold nanoparticle modified glass substrate.

Paper Details

Date Published: 19 February 2020
PDF: 4 pages
Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 112190D (19 February 2020); doi: 10.1117/12.2545872
Show Author Affiliations
Kota Koike, National Institute of Advanced Industrial Science and Technology (Japan)
Osaka Univ. (Japan)
Kazuki Bando, Osaka Univ. (Japan)
Jun Ando, Osaka Univ. (Japan)
Nicholas Smith, Osaka Univ. (Japan)
Kosuke Dodo, RIKEN (Japan)
Satoshi Kawata, Serendip Research Ltd. (Japan)
Mikiko Sodeoka, RIKEN (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 11219:
Visualizing and Quantifying Drug Distribution in Tissue IV
Kin Foong Chan; Conor L. Evans, Editor(s)

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