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Proceedings Paper

ElastoTweezers: A novel platform for high-precision cell elasticity measurements (Conference Presentation)

Paper Abstract

We developed a dual-beam optical tweezers setup with video-based force detection to precisely determine the mechanic properties of living cells in suspension with superior sensitivity compared to other techniques like optical stretchers and atomic force microscopy (AFM). With high time resolution we are able to determine both the elastic and the viscous properties of the cells. This novel setup is combined with custom-designed microfluidic cartridges to automatically and reliably pattern cells and beads at specific positions. The beads and cells are trapped and coupled to yield bead-cell-bead complexes. First results of the elasticity of HEK293 (human embryonic kidney) cells and skin fibroblasts are presented. The latter contain TMEM43-p.S358L mutation, which is linked to arrhythmogenic right ventricular cardiomyopathy.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124603 (9 March 2020); doi: 10.1117/12.2545809
Show Author Affiliations
Karsten Gall, Ionovation GmbH (Germany)
Andy Sischka, Ionovation GmbH (Germany)
Sebastian Knust, Univ. Bielefeld (Germany)
Hendrik Milting, Herz- und Diabeteszentrum Nordrhein-Westfalen (Germany)
Bastien Venzac, Univ. of Twente (Netherlands)
Séverine Le Gac, Univ. of Twente (Netherlands)
Elwin Vrouwe, Micronit Microfluidics B.V. (Netherlands)
Martina Viefhues, Univ. Bielefeld (Germany)
Dario Anselmetti, Univ. Bielefeld (Germany)

Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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