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Proceedings Paper

In vivo visualization of blood vessels in mouse ear by photoacoustic microscopy with transmissive liquid-crystal adaptive optics
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Paper Abstract

Photoacoustic microscopy (PAM) is a biological visualization technique that can provide high spatial resolution and high contrast images of deep structures in living tissues. However, because of the spherical aberration of the objective lens and the wavefront distortion due to the surface shape and light scattering of the specimen, obtained photoacoustic images in deep tissues are sometimes blurred or distorted. In order to solve this problem, we have developed a PAM using a transmissive liquid-crystal adaptive optics (AO) element. The transmissive and thin structure of the AO element can be easily installed in the PAM system. Using photoacoustic images of a USAF 1951 resolution test target measured through the glass substrate (thickness; 1.5-mm), the lateral resolutions in PAM were estimated with and without the AO element, when a flashlamp-pumped nanosecond pulse laser (pulse width, 5-ns; wavelength, 500-nm) and water-immersion objective lens (NA = 0.8) were employed. The lateral resolution of PAM at the depth of 1.5-mm was improved from 1.04 ± 0.04 μm to 0.53 ± 0.10 μm by optimizing AO corrections. We have also visualized small blood vessels in mouse ear in vivo by PAM with AO correction. Thus, by optimizing the AO correction according to the imaging depth, our proposed PAM improves the spatial resolution in biological tissues.

Paper Details

Date Published: 17 February 2020
PDF: 6 pages
Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 1124039 (17 February 2020); doi: 10.1117/12.2545777
Show Author Affiliations
Yusuke Notsuka, Saga Univ. (Japan)
Makoto Kurihara, Citizen Watch Co., Ltd. (Japan)
Nobuyuki Hashimoto, Citizen Watch Co., Ltd. (Japan)
Eiji Takahashi, Saga Univ. (Japan)
Yoshihisa Yamaoka, Saga Univ. (Japan)

Published in SPIE Proceedings Vol. 11240:
Photons Plus Ultrasound: Imaging and Sensing 2020
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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