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Proceedings Paper

Multiphoton imaging of dye penetration dynamics in tissue sections
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Paper Abstract

Rapid and in-depth labeling biological tissue samples is invaluable for studying tissue organization in three-dimensions. The current strategy in finding optimal labeling parameters for dye molecules through tissues often requires trial and error testing. Difficulty in establishing a standard approach is due to lack of information on the diffusion parameters of dye through tissues. In this study, we investigate the temporal progression of dye penetration in different tissue types. By the combination of multiphoton imaging of labeled tissues and application of the theoretical model of diffusion with well-defined boundary conditions, we aim to arrive at systematic parameters for optimizing dye penetration. Determination of the effective diffusion coefficient of the dyes used will then be useful for further application.

Paper Details

Date Published: 20 February 2020
PDF: 5 pages
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124428 (20 February 2020); doi: 10.1117/12.2545731
Show Author Affiliations
Po-Hang Tseng, National Chiao Tung Univ. (Taiwan)
Shu-Jen Chiang, National Taiwan Univ. (Taiwan)
Shean-Jen Chen, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Chiao Tung Univ. (Taiwan)


Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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