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Proceedings Paper

Development of fiber-based all-optical system for neurovascular coupling mechanism study using optogenetics
Author(s): Minkyung Kim; Hyun-joon Shin
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Paper Abstract

Optogenetics integrated with a variety of genetically encoded methods have been considered a very powerful technique for identifying neurovascular coupling mechanism. Recently, optogenetics has been applied to non-neuronal cells as well as neurons, to study the functions of various organs and tissues such as cardiovascular and bladder. However, the cerebrovascular network has a complicated environment in which cells and tissues with various features and functions coexist. In order to successfully apply optogenetics to study neurovascular coupling mechanism, it is necessary to develop not only optical stimulation techniques but also appropriate monitoring techniques. In addition, it has been very difficult to develop an appropriate optical system, since it is necessary to operate with the neurovascular network of a living animal maintained in the normal condition. Therefore, we have developed a fiber-based all-optical system that enables successful application of optogenetics to study neurovascular coupling mechanisms in living mouse. The developed optical system can perform rapid fluorescence imaging and optical stimulation at the same time, so it can measure various cell specific reactions and rapid blood flow that change after optical stimulation. In this study, we were able to observe changes in blood vessel diameter and blood flows caused by the cell activity of smooth muscle cells after selective optical stimulation using the developed all-optical system in SM22α-ChR2 transgenic mice. Also, the developed all-optical system can be used to study cerebrovascular disease or hemodynamics using optogenetics in vivo.

Paper Details

Date Published: 19 February 2020
PDF: 7 pages
Proc. SPIE 11227, Optogenetics and Optical Manipulation 2020, 112270B (19 February 2020); doi: 10.1117/12.2545680
Show Author Affiliations
Minkyung Kim, Korea Institute of Science and Technology (Korea, Republic of)
Korea Univ. of Science and Technology (Korea, Republic of)
Hyun-joon Shin, Korea Institute of Science and Technology (Korea, Republic of)
Korea Univ. of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 11227:
Optogenetics and Optical Manipulation 2020
Samarendra K. Mohanty; E. Duco Jansen; Anna W. Roe, Editor(s)

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