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Proceedings Paper

Correlation of metabolites in saliva and in vivo tissue of oral cancer patients based on fluorescence spectral deconvolution
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Paper Abstract

In vivo Native Fluorescence spectroscopic characterization of oral tissue and saliva of same group of normal, and patients with oral squamous cell carcinoma conditions were studied at 350 nm excitation. The measured fluorescence emission spectra exhibit broad emission with peaks due to NADH, FAD and Porphyrin. To resolve the emission from individual fluorophores, the measured fluorescence spectra were subjected to spectral deconvolution. Further, the variations in relative distribution, peak shifts and spectral broadening were analyzed with respect to the fluorophores, NADH, FAD, and porphyrin. The changes in the above photophysical characteristics of various native fluorophores between normal and cancer group in both tissue and saliva confirms that there is a significant molecular level changes during the transformation of normal into cancer. The extracted spectral signatures of tissues and saliva were also subjected to linear discriminant analysis and the diagnostic accuracy between tissue and saliva were compared.

Paper Details

Date Published: 21 February 2020
PDF: 8 pages
Proc. SPIE 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 112341D (21 February 2020); doi: 10.1117/12.2545662
Show Author Affiliations
Raja Pappu, Anna Univ., Chennai (India)
Yuvaraj Manoharan, SRM Art and Science College (India)
Einstein Gnanatheepam, Anna Univ., Chennai (India)
Sangeetha Ramamoorthy, Tamil Nadu Government Dental College and Hospital (India)
Prakasarao Aruna, Anna Univ., Chennai (India)
Jayachandran Sadaksharam, Tamil Nadu Government Dental College and Hospital (India)
Singaravelu Ganesan, Anna Univ., Chennai (India)


Published in SPIE Proceedings Vol. 11234:
Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos; Angela B. Seddon, Editor(s)

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