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Proceedings Paper

FLIm guided Raman imaging for detecting bovine pericardium cross-links and calcification (Conference Presentation)

Paper Abstract

We demonstrated the ability of fiber-based Fluorescence Lifetime Imaging (FLIm) guided Raman spectroscopy to monitor the quality of engineered vascular grafts with high speed and specificity. We report FLIm guided Raman imaging as an effective multimodal technique to evaluate scaffold cross-linking and localized calcification. Current results indicate that the lifetime of AR-BP shortens upon GA cross-linking,and Raman spectroscopy reveals secondary structural changes occurring in the Amide I region of cross-linked pericardia. GA fixed vascular grafts are prone to calcification, an effect linked to graft failure. The calcified regions exhibited shorter lifetimes in fluorescence spectral bands ranging from 380 to 455 nm and Raman spectra exhibited the specific hydroxyapatite signature at 960 cm-1 co-localized with these lower lifetime regions. We conclude that FLIm guided Raman imaging can detect cross-linking signatures and areas of calcification in tissue with biochemical specificity.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020, 112150I (9 March 2020); doi: 10.1117/12.2545632
Show Author Affiliations
Tanveer Ahmed Shaik, Leibniz-Institut für Photonische Technologien e.V. (Germany)
Alba Alfonso Garcia, Univ. of California, Davis (United States)
Xiangnan Zhou, Univ. of California, Davis (United States)
Anne K. Haudenschild, Univ. of California, Davis (United States)
Christoph Krafft, Leibniz-Institut für Photonische Technologien e.V. (Germany)
Laura Marcu, Univ. of California, Davis (United States)
Jürgen Popp, Leibniz-Institut für Photonische Technologien e.V. (Germany)


Published in SPIE Proceedings Vol. 11215:
Diagnostic and Therapeutic Applications of Light in Cardiology 2020
Kenton W. Gregory M.D.; Laura Marcu, Editor(s)

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