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Proceedings Paper

Time-gated fluorescence imaging and sensing using long lifetime near infrared quantum dots (Conference Presentation)
Author(s): Thomas Pons; Sophie Bouccara; Manon Debayle; Vincent Loriette; Nicolas Lequeux; Alexandra Fragola

Paper Abstract

Time-gated fluorescence imaging of near-infrared emitting ZnCuInSe/ZnS quantum dots (QDs) with fluorescence lifetimes in the range of 150−300 ns enables the efficient rejection of fast autofluorescence photons and the selection of QD fluorescence photons. This leads to complete elimination of autofluorescence background and a significant increase of imaging sensitivity. We demonstrate efficient detection and imaging of individual QD-labeled lymphoma cells circulating at mm/s velocities in blood vessels. In a second application, these QDs were used as a sensing platform to detect enzymatic activity using a ratiometric time-gated sensing scheme.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430W (9 March 2020); doi: 10.1117/12.2545627
Show Author Affiliations
Thomas Pons, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)
Sophie Bouccara, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)
Manon Debayle, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)
Vincent Loriette, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)
Nicolas Lequeux, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)
Alexandra Fragola, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France)


Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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