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Proceedings Paper

A spectral de-mixing model for triplex in vivo imaging of optical coherence tomography contrast agents
Author(s): Edwin Yuan; Peng Si; Saba Shevidi; Adam de la Zerda
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Paper Abstract

The ability to detect multiple contrast agents simultaneously would greatly enhance Optical Coherence Tomography (OCT) images, providing nuanced biological context to physiological structures. However, previous OCT contrast agent work has been limited to scenarios where only a single contrast agent could be robustly detected within each voxel. We present a novel spectroscopic technique for de-mixing the spectral signal from multiple OCT contrast agents within a single voxel. We validate our technique in vitro and also demonstrate in vivo imaging of three spectrally distinct gold nanobipyramids, trafficking within the lymphatic system of a live mouse. This approach opens the door to a much broader range of pre-clinical and clinical OCT applications where multiplexed labeling is desirable.

Paper Details

Date Published: 21 February 2020
PDF: 11 pages
Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 1122826 (21 February 2020); doi: 10.1117/12.2545501
Show Author Affiliations
Edwin Yuan, Stanford Univ. (United States)
Peng Si, Stanford Univ. (United States)
Saba Shevidi, Stanford Univ. (United States)
Adam de la Zerda, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 11228:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV
Joseph A. Izatt; James G. Fujimoto, Editor(s)

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