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Proceedings Paper

Hyper-raman spectroscopy of hydrogen bonding in DMSO-water mixtures
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Paper Abstract

Dimethyl sulfoxide (DMSO) is a biologically important solvent in part due to its dual miscibility with hydrophilic and hydrophobic molecules. Binary solutions of DMSO-water display non-ideal thermodynamics properties such as high viscosity and low freezing point due to hydrogen bonding. The unusual properties of DMSO-water solutions have been exploited to disrupt the formation of secondary structures of proteins during polymerase chain reaction assays and to act as a cryoprotectant for tissues. The exact coordination of the DMSO and water molecules remains unknown. Hyper- Raman scattering was employed for the first time to investigate binary systems of DMSO with water (H2O). As a part of this study, hyper-Raman and Raman spectra of pure solutions were first acquired and compared against existing Raman and IR spectroscopic data. Then the corresponding measurements were taken with deuterated DMSO-d6 and heavy water (D2O) to validate the analysis and to isolate overlapping spectral features. The permissive selection rules of hyper- Raman scattering provide new insight into disruptions of the self-hydrogen bonded networks of DMSO and water and the establishment of hydrogen bonded networks.

Paper Details

Date Published: 26 February 2020
PDF: 9 pages
Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 112521T (26 February 2020); doi: 10.1117/12.2545496
Show Author Affiliations
Xingqi Xu, Texas A&M Univ. (United States)
Zhejiang Univ. (China)
Christopher B. Marble, Texas A&M Univ. (United States)
Georgi I. Petrov, Texas A&M Univ. (United States)
Dawei Wang, Zhejiang Univ. (China)
Vladislav V. Yakovlev, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 11252:
Advanced Chemical Microscopy for Life Science and Translational Medicine
Ji-Xin Cheng; Wei Min; Garth J. Simpson, Editor(s)

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