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Proceedings Paper

G-protein coupled receptor signaling probed by a microtoroid-proteolipid system (Conference Presentation)

Paper Abstract

Most biosensors rely on immobilized antibodies or aptamers. In contrast, receptor proteins exist naturally in lipid bilayers and are highly specific to small molecules. We use a frequency-locked optical whispering evanescent resonator (FLOWER) system for real-time quantification of rhodopsin incorporation into an artificial lipid membrane and observe photo-induced molecular transformations upon light activation. Our study of proteolipid membrane coated microtoroids for probing the local activity of G-protein coupled receptors was further expanded to kappa-opioid receptors and their endogenous ligand Dynorphin-A. G-protein coupled receptor signaling probed by a microtoroid-proteolipid system will facilitate drug discovery and therapeutic interventions.

Paper Details

Date Published: 9 March 2020
PDF
Proc. SPIE 11233, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XX, 112330M (9 March 2020); doi: 10.1117/12.2545495
Show Author Affiliations
Phuong Diem Nguyen, The Univ. of Arizona (United States)
Adley Gin, The Univ. of Arizona (United States)
Cheng Li, The Univ. of Arizona (United States)
Judith Su, The Univ. of Arizona (United States)


Published in SPIE Proceedings Vol. 11233:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XX
Israel Gannot, Editor(s)

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