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Proceedings Paper

Rapid detection of antibiotic-resistant bacteria at the single-cell level using two-photon excitation fluorescence and coherent anti-Stokes Raman scattering microscopy (Conference Presentation)

Paper Abstract

The current standard for antibiotic susceptibility testing (AST) is based on measuring bacterial growth after 10-24 hours of proliferation. Considering that many life-threatening conditions of infection exist, rapid AST techniques are urgently needed. We developed a rapid AST method based on two-photon fluorescence and coherent anti-Stokes Raman scattering microscopy which can detect antibiotic responses of bacteria within one hour. We used Pseudomonas aeruginosa as a representative pathogen model, and found that antibiotic treatment greatly reduces nicotinamide adenine dinucleotide (phosphate) levels in the bacteria. This enables rapid determination of bacterial susceptibility at the single cell level.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230B (10 March 2020); doi: 10.1117/12.2545438
Show Author Affiliations
Chi Zhang, Univ. of Illinois (United States)
Jungeun Won, Univ. of Illinois (United States)
Stephen A. Boppart, Univ. of Illinois (United States)


Published in SPIE Proceedings Vol. 11223:
Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
Tianhong Dai; Jürgen Popp; Mei X. Wu M.D., Editor(s)

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