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Proceedings Paper

Dynamic signatures of lipid droplets reveal cellular metabolic changes associated with stimuli and drug treatments (Conference Presentation)

Paper Abstract

Coherent anti-Stokes Raman scattering (CARS) microscopy was used to disclose dynamic information from lipid droplets (LDs) in living cancer cells. Statistical analyses of LD dynamics show different pools of LDs associated with LD synthesis and degradation processes. We found that each LD population satisfies a log-normal distribution, thus allowing quantitative comparison through fitting of population histograms. Two-dimensional analyses of LD displacements and speeds offer dynamic signatures of cells to monitor responses to stimuli and drugs. We revealed hypothermia-induced cellular metabolic changes and a two-step metabolic response during apoptosis. We also explored dynamic signatures of LDs during starvation and various drug exposures.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430V (9 March 2020); doi: 10.1117/12.2545387
Show Author Affiliations
Chi Zhang, Univ. of Illinois (United States)
Stephen A. Boppart, Univ. of Illinois (United States)

Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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