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Proceedings Paper

Optimizing selective phototherapy of port wine stain by two-photon fluorescence and optical coherence tomography imaging (Conference Presentation)
Author(s): Iván Coto Hernández; Nate Jowett; Tan Oon

Paper Abstract

A port-wine stain is a congenital vascular malformation, which may worsen if left untreated. Selective photothermolysis with Pulse-dye lasers is a well-established treatment for PWS. However, outcomes are often unpredictable due to tissue heterogeneity. This study characterizes the three-dimensional architecture of ex vivo human skin from control and PWS regions of five patients before and after laser treatment using optical coherence tomography (OCT), two-photon excitation fluorescence microscopy (TPEM), and second harmonic generation (SHG). Results suggest that combining the high penetration depth of OCT with the high resolution of multiphoton imaging could provide a means for real-time guidance of selective phototherapy.

Paper Details

Date Published: 6 March 2020
Proc. SPIE 11211, Photonics in Dermatology and Plastic Surgery 2020, 112110I (6 March 2020); doi: 10.1117/12.2545320
Show Author Affiliations
Iván Coto Hernández, Harvard Medical School (United States)
Nate Jowett, Harvard Medical School (United States)
Tan Oon, Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 11211:
Photonics in Dermatology and Plastic Surgery 2020
Bernard Choi; Haishan Zeng, Editor(s)

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