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Proceedings Paper

Label-free multispectral monitoring of functional state: from embryos to human articular cartilage tissue (Conference Presentation)

Paper Abstract

Automated, unbiased methods of non-invasive cell monitoring able to deal with complex biological heterogeneity are fundamentally important for biomedical research. Label-free imaging provides information about endogenous autofluorescent metabolites, enzymes and cofactors. Our multispectral fluorescence imaging technique allows precise quantification of native fluorophores in cells and tissues. This study uses label-free multispectral analysis to extract different fluorophores and redox ratio from single cells (oocytes, cultured cancer cells) as well as blastocyst embryos. Additionally, we characterise the molecular composition, structure and functional status of ex vivo healthy bovine and osteoarthritic human knee articular cartilage to assess 2 types of experimental treatments.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112510H (10 March 2020); doi: 10.1117/12.2545265
Show Author Affiliations
Saabah B. Mahbub, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Jared M. Campbell, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Tiffany C. Y. Tan, The Univ. of Adelaide (Australia)
ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Kylie R. Dunning, The Univ. of Adelaide (Australia)
ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)
Abbas Habibalahi, The Univ. of New South Wales (Australia)
Michael Bertoldo, The Univ. of New South Wales (Australia)
Dale Goss, The Univ. of New South Wales (Australia)
Lindsay Wu, The Univ. of New South Wales (Australia)
Ewa M. Goldys, The Univ. of New South Wales (Australia)
ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia)


Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

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