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Proceedings Paper

Fiber-based instrument for simultaneous exogenous fluorescence and endogenous fluorescence lifetime imaging of engineered vascular tissue (Conference Presentation)
Author(s): Cai Li; Alba Alfonso García; Lauren Uyesaka; Benjamin E. Sherlock; Leigh G. Griffiths; Laura Marcu

Paper Abstract

Endogenous and exogenous fluorescence imaging have shown great values for monitoring and studying all kind of biological processes including vascular tissue regeneration and disease progression. In this study, we present a fiber-based optical imaging instrument that is able to simultaneously acquire endogenous and exogenous fluorescence images from tissue samples using a reflective optical chopper wheel to temporally interleave the two modalities. The functionality of the system was demonstrated by imaging native tissue constructs seeded with cells labeled with different dyes. The cellular regions were clearly resolved in both modalities, providing an exogenous and in-situ validation for endogenous fluorescence lifetime images.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 1124313 (9 March 2020); doi: 10.1117/12.2545251
Show Author Affiliations
Cai Li, Univ. of California, Davis (United States)
Alba Alfonso García, Univ. of California, Davis (United States)
Lauren Uyesaka, Univ. of California, Davis (United States)
Benjamin E. Sherlock, Univ. of California, Davis (United States)
Leigh G. Griffiths, Mayo Clinic (United States)
Laura Marcu, Univ. of California, Davis (United States)


Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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