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Proceedings Paper

Quantitative phase imaging of neuronal movement during action potential (Conference Presentation)

Paper Abstract

Nanometer-scale deformations of the neuron accompany the action potential. These displacements are measured using a fast quantitative phase microscope and averaged in synchrony with optogenetic stimulation of cultured neurons. The phase movie is further processed by leveraging the spatial and temporal distribution of the spiking signal to detect and segment the separate action potentials in individual cells. An accompanying confocal fluorescence microscopy provides the 3-D cell shape for calibration of the refractive index to calculate the mechanical displacements from the optical phase. Together, these results illuminate the underlying mechanism of the cellular deformations and techniques for achieving all-optical single spike detection.

Paper Details

Date Published: 11 March 2020
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Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490S (11 March 2020); doi: 10.1117/12.2545250
Show Author Affiliations
Kevin C. Boyle, Stanford Univ. (United States)
Tong Ling, Stanford Univ. (United States)
Valentina Zuckerman, Stanford Univ. (United States)
Thomas Flores, Stanford Univ. (United States)
Daniel V. Palanker, Stanford Univ. (United States)


Published in SPIE Proceedings Vol. 11249:
Quantitative Phase Imaging VI
Yang Liu; Gabriel Popescu; YongKeun Park, Editor(s)

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