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Proceedings Paper

Quantitative fluorescence spectroscopy to detect and identify bacteria and to monitor their viability (Conference Presentation)
Author(s): Frédérique Vanholsbeeck; Julia Robertson; Fang Ou; Claire Honney; Rachel Guo; Simon Swift; Cushla M. McGoverin

Paper Abstract

Detecting and characterising bacteria as well as monitoring their viability are routine tasks in microbiology. Conventional methods of cell detection and viability monitoring are often time-consuming and/or expensive. We have developed a near-real time, cost-effective and portable fluorometer, the optrode, for quantifying fluorescence signals. We are currently developing protocols to use the optrode to detect, identify and quantify bacteria as well as to monitor their viability using nucleic acid stains such as SYTO 9 and propidium iodide that are routinely used as live/dead stains. Our results are promising but indicate that better dyes are needed to fully characterise bacteria.

Paper Details

Date Published: 10 March 2020
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Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 1122304 (10 March 2020); doi: 10.1117/12.2545243
Show Author Affiliations
Frédérique Vanholsbeeck, The Univ. of Auckland (New Zealand)
Julia Robertson, The Univ. of Auckland (New Zealand)
Fang Ou, The Univ. of Auckland (New Zealand)
Claire Honney, The Univ. of Auckland (New Zealand)
Rachel Guo, The Univ. of Auckland (New Zealand)
Simon Swift, The Univ. of Auckland (New Zealand)
Cushla M. McGoverin, The Univ. of Auckland (New Zealand)


Published in SPIE Proceedings Vol. 11223:
Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020
Tianhong Dai; Jürgen Popp; Mei X. Wu M.D., Editor(s)

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